Integrative genomic and proteomic analysis of prostate cancer reveals signatures of metastatic progression.

TitleIntegrative genomic and proteomic analysis of prostate cancer reveals signatures of metastatic progression.
Publication TypeJournal Article
Year of Publication2005
AuthorsVarambally S, Yu J, Laxman B, Rhodes DR, Mehra R, Tomlins SA, Shah RB, Chandran U, Monzon FA, Becich MJ, Wei JT, Pienta KJ, Ghosh D, Rubin MA, Chinnaiyan AM
JournalCancer Cell
Volume8
Issue5
Pagination393-406
Date Published2005 Nov
ISSN1535-6108
KeywordsDisease Progression, Gene Expression Profiling, Humans, Immunoblotting, Male, Neoplasm Metastasis, Neoplasm Proteins, Prognosis, Prostatic Neoplasms, Protein Array Analysis, RNA, Messenger
Abstract

Molecular profiling of cancer at the transcript level has become routine. Large-scale analysis of proteomic alterations during cancer progression has been a more daunting task. Here, we employed high-throughput immunoblotting in order to interrogate tissue extracts derived from prostate cancer. We identified 64 proteins that were altered in prostate cancer relative to benign prostate and 156 additional proteins that were altered in metastatic disease. An integrative analysis of this compendium of proteomic alterations and transcriptomic data was performed, revealing only 48%-64% concordance between protein and transcript levels. Importantly, differential proteomic alterations between metastatic and clinically localized prostate cancer that mapped concordantly to gene transcripts served as predictors of clinical outcome in prostate cancer as well as other solid tumors.

DOI10.1016/j.ccr.2005.10.001
Alternate JournalCancer Cell
PubMed ID16286247
Grant List5P30 CA46592 / CA / NCI NIH HHS / United States
P50CA69568 / CA / NCI NIH HHS / United States
R01 GM072007 / GM / NIGMS NIH HHS / United States
R01 GM072007-03 / GM / NIGMS NIH HHS / United States
R01AG21404 / AG / NIA NIH HHS / United States
R01GM72007-01 / GM / NIGMS NIH HHS / United States
UO1 CA111275-01 / CA / NCI NIH HHS / United States